Application

Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected RecQ1 in human kidney and human tonsil tissue sections.

Anti-RecQ1, clone 5A12.1, Cat. No. MABE1055, is a highly specific mouse monoclonal antibody that targets RecQ1 and has been tested for use in Western Blotting and Immunohistochemistry (Paraffin).

General description

ATP-dependent DNA helicase Q1 (UniProt: P46063; also known as EC:3.6.4.12, DNA helicase, RecQ-like type 1, RecQ1, DNA-dependent ATPase Q1, RecQ protein-like 1) is encoded by the RECQL (also known as RECQ1, RECQL1) gene (Gene ID: 5965) in human. RecQ helicases are a ubiquitous family of DNA unwinding enzymes involved in the maintenance of chromosome stability. They participate in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Five members of the RecQ family have been reported in human cells: BLM, RECQ1, RECQ4, RECQ5, and WRN. RecQ1 displays high expression in heart, lung, skeletal muscle and kidney. It is also highly expressed in tumor cells and although brain exhibits low level of EecQ1, its expression is dramatically increased in human glioblastoma. Depletion of RecQ1 affects proliferation of glioblastoma cells and causes an increase in the level of DNA damage. RecQ1 depleted tumor cells are shown to be more sensitive to anti-tumor agents, such as hydroxyurea and temozolomide.

Immunogen

GST/His-tagged recombinant fragment corresponding to 87 amino acids from the internal region of human ATP-dependent DNA helicase Q1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RecQ1 in Raji cell lysate.

Specificity

Clone 5A12.1 specifically detects human ATP-dependent DNA helicase Q1 (RecQ1). It targets an epitope within 87 amino acids from the internal region.

Target description

~73 kDa observed; 73.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).